Mouse anti Tubulin beta 3 antibody, clone TU-20 recognizes class III beta-tubulin which is reported to be restricted to neuronal tissue. Mouse anti Tubulin beta 3 antibody, clone TU-20 has been used to investigate tumors of neuronal origin including neuroblastoma and ganglioneuroma. Class III beta tubulin has not been detected in tumors of non neuronal origin. In particular, beta III tubulin’s expression correlates with the earliest phases of neuronal differentiation. For this reason, beta tubulin III has implications in neurogenesis, axon guidance and maintenance. Since it was discovered that the human brain generates new neurons from stem cell pools, beta tubulin III has been used as a marker of. Select β 3 Tubulin antibodies from monoclonal antibodies listed below. View detailed β 3 Tubulin antibody specifications by linking to the specific product blocks. Select appropriate β 3 Tubulin antibodies for your research by isotype, epitope, applications and species reactivity. 04.12.2019 · The expression of class III beta-tubulin was not related to retinal cell morphology or cell function, but rather to the cell lineage, suggesting that the expression of beta-tubulin III may be due to the retinal cell specific developmental program. A transgenic mouse class-III beta tubulin reporter using yellow fluorescent protein.
In adults, tubulin beta 3 TUBB3 is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 TUBB3 is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors. ab7751 staining beta III Tubulin in induced neurons derived from human pluripotent cells by ICC/IF Immunocytochemistry/immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody 1/500 for 16 hours at 4°C. Tubulin is the main component of microtubules. In adults, tubulin beta 3 TUBB3 is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 TUBB3 is. Use the list below to choose the beta-III Tubulin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our beta-III Tubulin polyclonal antibodies and browse our beta-III Tubulin monoclonal antibody catalog.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased. β-Tubulin即微管蛋白，是细胞的一种骨架蛋白。Tubulin分为α、β、γ、δ、ε等多种tubulin，其中α-Tubulin和β-Tubulin可以形成异源二聚体，是形成微管的最主要的两种Tubulin。α-tubulin和β-tubulin分子量分别为55kDa和50kDa，其实际检测条带均在55kDa左右。作为内参抗体，Abbkine的β-tubulin抗体的蛋白水平通常不会. β3 Tubulin Antibody 2G10 is a mouse monoclonal IgG 2a kappa light chain provided at 200 µg/ml; raised against β3 Tubulin of rat origin, with epitope mapping to amino acids 436-450; beta 3 Tubulin Antibody 2G10 is recommended for detection of neuronal specific β3 Tubulin of mouse, rat, human and bovine origin by WB, IP, IF and IHCP.
Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Erfahren Sie mehr über Anti-beta 3-Tubulin Rabbit Polyclonal Antibody. "We enable Science" - durch eine große Produktauswahl, exzellente Prozesse und unsere kompetenten Mitarbeiter. beta Tubulin 3/ Tuj1 antibody [GT886] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Tubulin 3/ Tuj1 protein stained by beta Tubulin 3/ Tuj1 antibody [GT886] GTX631830 diluted at 1:500.
Beta-Tubulin, is usually used as loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. OriGene offers the best quality of Anti-beta Tubulin antibodies for Western Blot. beta 3 Tubulin Polyclonal Antibody Formalin-fixed and paraffin embedded rat brain labeled with Anti-TUBB3 Polyclonal Antibody, Unconjugated bs-4512R at 1:200 followed by conjugation to the secondary antibody and DAB staining. Beta III Tubulin is a reported alias name for the human gene TUBB3, or 'tubulin beta 3 class III'. The 450-amino acid protein is a member of the Tubulin family. The cellular localization is predicted to be cytoplasmic. The Beta III Tubulin antigen is a documented neuronal marker. See the supplier page to learn important antibody details, such. Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins and extracts from CAD cells using α-Tubulin Antibody 2144left, β-Tubulin. 302 302: β3-Tubulin: rabbit polyclonal, antiserum: WB IP ICC IHC IHC-P: 200µl: 350.00: 302 304: β3-Tubulin: Guinea pig polyclonal, antiserum: WB IP ICC IHC IHC-P.
Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Kaninchen Polyklonal TUBB3 Antikörper AA 222-438 für ICC, IHC, WB. Order anti-TUBB3 Antikörper ABIN1860885. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human NP_AAL28094, NCBI and rat AAM28438, NCBI protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified.
TUBB3 ELISA Kit Human für Cell Lysate, Tissue Homogenate. Order TUBB3 ELISA Kit ABIN420333. Sullivan and Cleveland 1986 isolated a partial TUBB3 cDNA, which they designated beta-4, from a human placenta cDNA library. Ranganathan et al. 1998 cloned cDNAs encoding human TUBB3 from a prostate carcinoma cell line. Wang et al. 1986 cloned mouse Tubb3, which they called M-beta-3, from testis and bone marrow cDNA libraries. Northern blot analysis detected abundant Tubb3 expression in. Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB. - Find MSDS or SDS, a COA, data sheets and more information.
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 Similar to TUJ1 Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 Similar to TUJ1 is an antibody against Tubulin for use in ELISA, IC, IH, IHP, IP & WB with more than 55 product citations. - Find MSDS or SDS, a COA, data sheets and more information.
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